GB 4789.40-2016 Translated English of Chinese Standard. GB4789.40-2016: National Food Safety Standard - Food Microbiological Examination - Examination of Cronobacter (Enterobacter Sakazakii)https://www.chinesestandard.net, 2017 M11 4 - 14 pages This Standard specifies the examination method for Cronobacter in food. This Standard applies to the examination of Cronobacter in infant formula, milk and dairy products and their raw materials. |
Contents
| 3 | |
Scope | 4 |
Medium and reagent | 5 |
Operation steps | 6 |
Result and report | 7 |
Operation steps | 8 |
Annex A Medium and reagent | 9 |
Annex B The most probable number MPN search table for Cronobacter | 14 |
Common terms and phrases
0.015 g Distilled 1.0 g Bromocresol 3.0 g Glucose 5.0 g Yeast add into 900 adjust pH Agar amygdalin Autoclave at 121°C Bromocresol purple 0.015 Buffer peptone water chloride 5.0 g Culture at 30°C Culture at 36°C D-sucrose decarboxylase test Dipotassium phosphate dissolve the components Distilled water 1000 Examination of Cronobacter Experimental method Inoculate extract 3.0 g Food Microbiological Examination g Bromocresol purple g Dipotassium g mL g Potassium dihydrogen g Sodium g Yeast extract Glucose 1.0 Glucose 1.0 g Heat and dissolve Inoculate the culture L-lysine decarboxylase medium L-ornithine decarboxylase medium lauryl sulfate tryptose liquid level liquid medium medium is purple mL of BPW mLST-Vm broth Modified lauryl sulfate monohydrochloride 5.0 negative is yellow Observe the results oxidase test phosphate 1.0 g phosphate 2.75 g pipette Potassium dihydrogen phosphate preheated to 44°C Preparation method Heat Sodium chloride 5.0 Sub-packaging 5 mL test is positive Tryptone tube vancomycin solution Yeast extract 3.0